Statistical evaluation of the medium components for the production of high biomass, α-amylase and protease enzymes by Piriformospora indica using Plackett-Burman experimental design.

ABSTRACT: Piriformospora indica, a member of basidiomycota is an axenically cultivable endophytic fungus which exerts plant growth promoting effects on its host plant. P. indica is known to produce α-amylase and protease. Since the organism exhibits beneficial role in plant growth promotion, achieving high biomass is immensely essential. Hence to enable the commercial production, screening of medium components is a necessary step. The present paper investigates the screening of medium components using Plackett-Burman experimental design wherein the parameters such as α-amylase, protease and biomass have been examined. The parameters α-amylase, protease and biomass was found to vary from 0.25 to 0.45 mg^-1 ml^-1 min^-1, 0.1 to 0.15 mg^-1 ml^-1 h^-1 and 0.8 to 22.6 g l^-1, respectively, in 16 runs which demonstrates the strong influence of the medium components.

SUBMITTER: Swetha S 

PROVIDER: S-EPMC4145629 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature