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Pantoea sp. isolated from tropical fresh water exhibiting N-acyl homoserine lactone production.

ABSTRACT: N-Acyl homoserine lactone (AHL) serves as signaling molecule for quorum sensing (QS) in Gram-negative bacteria to regulate various physiological activities including pathogenicity. With the aim of isolating freshwater-borne bacteria that can cause outbreak of disease in plants and portrayed QS properties, environmental water sampling was conducted. Here we report the preliminary screening of AHL production using Chromobacterium violaceum CV026 and Escherichia coli [pSB401] as AHL biosensors. The 16S rDNA gene sequence of isolate M009 showed the highest sequence similarity to Pantoea stewartii S9-116, which is a plant pathogen. The isolated Pantoea sp. was confirmed to produce N-3-oxohexanoyl-L-HSL (3-oxo-C6-HSL) through analysis of high resolution mass tandem mass spectrometry.

SUBMITTER: Tan WS

PROVIDER: S-EPMC4146356 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Pantoea sp. isolated from tropical fresh water exhibiting N-acyl homoserine lactone production.

Tan Wen-Si WS Muhamad Yunos Nina Yusrina NY Tan Pui-Wan PW Mohamad Nur Izzati NI Adrian Tan-Guan-Sheng TG Yin Wai-Fong WF Chan Kok-Gan KG

TheScientificWorldJournal 20140812

N-Acyl homoserine lactone (AHL) serves as signaling molecule for quorum sensing (QS) in Gram-negative bacteria to regulate various physiological activities including pathogenicity. With the aim of isolating freshwater-borne bacteria that can cause outbreak of disease in plants and portrayed QS properties, environmental water sampling was conducted. Here we report the preliminary screening of AHL production using Chromobacterium violaceum CV026 and Escherichia coli [pSB401] as AHL biosensors. The ...[more]

PMID: 25197715

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