Ontology highlight
ABSTRACT:
SUBMITTER: Yang Y
PROVIDER: S-EPMC4148141 | biostudies-literature | 2014 Aug
REPOSITORIES: biostudies-literature
Yang Yi Y Qin Yangzhong Y Ding Qing Q Bakhtina Marina M Wang Lijuan L Tsai Ming-Daw MD Zhong Dongping D
Biochemistry 20140815 33
DNA polymerases slide on DNA during replication, and the interface must be mobile for various conformational changes. The role of lubricant interfacial water is not understood. In this report, we systematically characterize the water dynamics at the interface and in the active site of a tight binding polymerase (pol β) in its binary complex and ternary state using tryptophan as a local optical probe. Using femtosecond spectroscopy, we observed that upon DNA recognition the surface hydration wate ...[more]