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High-resolution structure determination by continuous-rotation data collection in MicroED.

ABSTRACT: MicroED uses very small three-dimensional protein crystals and electron diffraction for structure determination. We present an improved data collection protocol for MicroED called 'continuous rotation'. Microcrystals are continuously rotated during data collection, yielding more accurate data. The method enables data processing with the crystallographic software tool MOSFLM, which resulted in improved resolution for the model protein lysozyme. These improvements are paving the way for the broad implementation and application of MicroED in structural biology.

SUBMITTER: Nannenga BL

PROVIDER: S-EPMC4149488 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature

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High-resolution structure determination by continuous-rotation data collection in MicroED.

Nannenga Brent L BL Shi Dan D Leslie Andrew G W AGW Gonen Tamir T

Nature methods 20140803 9

MicroED uses very small three-dimensional protein crystals and electron diffraction for structure determination. We present an improved data collection protocol for MicroED called 'continuous rotation'. Microcrystals are continuously rotated during data collection, yielding more accurate data. The method enables data processing with the crystallographic software tool MOSFLM, which resulted in improved resolution for the model protein lysozyme. These improvements are paving the way for the broad ...[more]

PMID: 25086503

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