Fragment-based determination of a proteinase K structure from MicroED data using ARCIMBOLDO_SHREDDER.

ABSTRACT: Structure determination of novel biological macromolecules by X-ray crystallography can be facilitated by the use of small structural fragments, some of only a few residues in length, as effective search models for molecular replacement to overcome the phase problem. Independence from the need for a complete pre-existing model with sequence similarity to the crystallized molecule is the primary appeal of ARCIMBOLDO, a suite of programs which employs this ab initio algorithm for phase determination. Here, the use of ARCIMBOLDO is investigated to overcome the phase problem with the electron cryomicroscopy (cryoEM) method known as microcrystal electron diffraction (MicroED). The results support the use of the ARCIMBOLDO_SHREDDER pipeline to provide phasing solutions for a structure of protein

SUBMITTER: Richards LS 

PROVIDER: S-EPMC7397493 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature