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Efficient and specific generation of knockout mice using Campylobacter jejuni CRISPR/Cas9 system.


ABSTRACT: The Streptococcus pyogenes CRISPR/Cas9 (SpCas9) system is now widely utilized to generate genome engineered mice; however, some studies raised issues related to off-target mutations with this system. Herein, we utilized the Campylobacter jejuni Cas9 (CjCas9) system to generate knockout mice. We designed sgRNAs targeting mouse Tyr or Foxn1 and microinjected into zygotes along with CjCas9 mRNA. We obtained newborn mice from the microinjected embryos and confirmed that 50% (Tyr) and 38.5% (Foxn1) of the newborn mice have biallelic mutation on the intended target sequences, indicating efficient genome targeting by CjCas9. In addition, we analyzed off-target mutations in founder mutant mice by targeted deep sequencing and whole genome sequencing. Both analyses revealed no off-target mutations at potential off-target sites predicted in silico and no unexpected random mutations in analyzed founder animals. In conclusion, the CjCas9 system can be utilized to generate genome edited mice in a precise manner.

SUBMITTER: Lee JY 

PROVIDER: S-EPMC7118303 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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Efficient and specific generation of knockout mice using <i>Campylobacter jejuni</i> CRISPR/Cas9 system.

Lee Jae Young JY   Jang Yoo Jin YJ   Bae Ji Hyun JH   Lee Yoon Hoo YH   Bae Hee Sook HS   Kim Seokjoong S   Park Sin-Gi SG   Koo Ok Jae OJ   Yeom Su Cheong SC  

Biochemistry and biophysics reports 20200401


The <i>Streptococcus pyogenes</i> CRISPR/Cas9 (SpCas9) system is now widely utilized to generate genome engineered mice; however, some studies raised issues related to off-target mutations with this system. Herein, we utilized the <i>Campylobacter jejuni</i> Cas9 (CjCas9) system to generate knockout mice. We designed sgRNAs targeting mouse <i>Tyr</i> or <i>Foxn1</i> and microinjected into zygotes along with CjCas9 mRNA. We obtained newborn mice from the microinjected embryos and confirmed that 5  ...[more]

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