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Metabolic phenotyping reveals a lipid mediator response to ionizing radiation.

ABSTRACT: Exposure to ionizing radiation has dramatically increased in modern society, raising serious health concerns. The molecular response to ionizing radiation, however, is still not completely understood. Here, we screened mouse serum for metabolic alterations following an acute exposure to ? radiation using a multiplatform mass-spectrometry-based strategy. A global, molecular profiling revealed that mouse serum undergoes a series of significant molecular alterations following radiation exposure. We identified and quantified bioactive metabolites belonging to key biochemical pathways and low-abundance, oxygenated, polyunsaturated fatty acids (PUFAs) in the two groups of animals. Exposure to ? radiation induced a significant increase in the serum levels of ether phosphatidylcholines (PCs) while decreasing the levels of diacyl PCs carrying PUFAs. In exposed mice, levels of pro-inflammatory, oxygenated metabolites of arachidonic acid increased, whereas levels of anti-inflammatory metabolites of omega-3 PUFAs decreased. Our results indicate a specific serum lipidomic biosignature that could be utilized as an indicator of radiation exposure and as novel target for therapeutic intervention. Monitoring such a molecular response to radiation exposure might have implications not only for radiation pathology but also for countermeasures and personalized medicine.

SUBMITTER: Laiakis EC

PROVIDER: S-EPMC4156265 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature

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Metabolic phenotyping reveals a lipid mediator response to ionizing radiation.

Laiakis Evagelia C EC Strassburg Katrin K Bogumil Ralf R Lai Steven S Vreeken Rob J RJ Hankemeier Thomas T Langridge James J Plumb Robert S RS Fornace Albert J AJ Astarita Giuseppe G

Journal of proteome research 20140815 9

Exposure to ionizing radiation has dramatically increased in modern society, raising serious health concerns. The molecular response to ionizing radiation, however, is still not completely understood. Here, we screened mouse serum for metabolic alterations following an acute exposure to γ radiation using a multiplatform mass-spectrometry-based strategy. A global, molecular profiling revealed that mouse serum undergoes a series of significant molecular alterations following radiation exposure. We ...[more]

PMID: 25126707

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Metabolic phenotyping reveals a lipid mediator response to ionizing radiation.

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Metabolic phenotyping reveals a lipid mediator response to ionizing radiation.

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