Analytic 3D imaging of mammalian nucleus at nanoscale using coherent x-rays and optical fluorescence microscopy.
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ABSTRACT:
SUBMITTER: Song C
PROVIDER: S-EPMC4156684 | biostudies-literature | 2014 Sep
REPOSITORIES: biostudies-literature
Publications
Analytic 3D imaging of mammalian nucleus at nanoscale using coherent x-rays and optical fluorescence microscopy.
Song Changyong C Takagi Masatoshi M Park Jaehyun J Xu Rui R Gallagher-Jones Marcus M Imamoto Naoko N Ishikawa Tetsuya T
Biophysical journal 20140901 5
Despite the notable progress that has been made with nano-bio imaging probes, quantitative nanoscale imaging of multistructured specimens such as mammalian cells remains challenging due to their inherent structural complexity. Here, we successfully performed three-dimensional (3D) imaging of mammalian nuclei by combining coherent x-ray diffraction microscopy, explicitly visualizing nuclear substructures at several tens of nanometer resolution, and optical fluorescence microscopy, cross confirmin ...[more]
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