Ontology highlight
ABSTRACT:
SUBMITTER: Gee JM
PROVIDER: S-EPMC4156920 | biostudies-literature | 2014 Sep
REPOSITORIES: biostudies-literature
Gee J Michael JM Smith Nathan A NA Fernandez Fernando R FR Economo Michael N MN Brunert Daniela D Rothermel Markus M Morris S Craig SC Talbot Amy A Palumbos Sierra S Ichida Jennifer M JM Shepherd Jason D JD West Peter J PJ Wachowiak Matt M Capecchi Mario R MR Wilcox Karen S KS White John A JA Tvrdik Petr P
Neuron 20140821 5
New strategies for introducing genetically encoded activity indicators into animal models facilitate the investigation of nervous system function. We have developed the PC::G5-tdT mouse line that expresses the GCaMP5G calcium indicator in a Cre-dependent fashion. Instead of targeting the ROSA26 locus, we inserted the reporter cassette nearby the ubiquitously expressed Polr2a gene without disrupting locus integrity. The indicator was tagged with IRES-tdTomato to aid detection of positive cells. T ...[more]