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ABSTRACT:
SUBMITTER: Toccafondi M
PROVIDER: S-EPMC4157429 | biostudies-literature | 2014 Sep
REPOSITORIES: biostudies-literature
Acta crystallographica. Section F, Structural biology communications 20140827 Pt 9
Glucose-1-phosphate uridylyltransferase from Erwinia amylovora CFPB1430 was expressed as a His-tag fusion protein in Escherichia coli. After tag removal, the purified protein was crystallized from 100 mM Tris pH 8.5, 2 M ammonium sulfate, 5% ethylene glycol. Diffraction data sets were collected to a maximum resolution of 2.46 Å using synchrotron radiation. The crystals belonged to the hexagonal space group P62, with unit-cell parameters a = 80.67, b = 80.67, c = 169.18. The structure was solved ...[more]