Dew inspired breathing-based detection of genetic point mutation visualized by naked eye.
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ABSTRACT:
SUBMITTER: Xie L
PROVIDER: S-EPMC4158330 | biostudies-literature | 2014 Sep
REPOSITORIES: biostudies-literature
Publications
Dew inspired breathing-based detection of genetic point mutation visualized by naked eye.
Xie Liping L Wang Tongzhou T Huang Tianqi T Hou Wei W Huang Guoliang G Du Yanan Y
Scientific reports 20140909
A novel label-free method based on breathing-induced vapor condensation was developed for detection of genetic point mutation. The dew-inspired detection was realized by integration of target-induced DNA ligation with rolling circle amplification (RCA). The vapor condensation induced by breathing transduced the RCA-amplified variances in DNA contents into visible contrast. The image could be recorded by a cell phone for further or even remote analysis. This green assay offers a naked-eye-reading ...[more]
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