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Aqueous oxidative Heck reaction as a protein-labeling strategy.


ABSTRACT: An increasing number of chemical reactions are being employed for bio-orthogonal ligation of detection labels to protein-bound functional groups. Several of these strategies, however, are limited in their application to pure proteins and are ineffective in complex biological samples such as cell lysates. Here we present the palladium-catalyzed oxidative Heck reaction as a new and robust bio-orthogonal strategy for linking functionalized arylboronic acids to protein-bound alkenes in high yields and with excellent chemoselectivity even in the presence of complex protein mixtures from living cells. Advantageously, this reaction proceeds under aerobic conditions, whereas most other metal-catalyzed reactions require inert atmosphere.

SUBMITTER: Ourailidou ME 

PROVIDER: S-EPMC4159585 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature

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Aqueous oxidative Heck reaction as a protein-labeling strategy.

Ourailidou Maria Eleni ME   van der Meer Jan-Ytzen JY   Baas Bert-Jan BJ   Jeronimus-Stratingh Margot M   Gottumukkala Aditya L AL   Poelarends Gerrit J GJ   Minnaard Adriaan J AJ   Dekker Frank J FJ  

Chembiochem : a European journal of chemical biology 20131220 2


An increasing number of chemical reactions are being employed for bio-orthogonal ligation of detection labels to protein-bound functional groups. Several of these strategies, however, are limited in their application to pure proteins and are ineffective in complex biological samples such as cell lysates. Here we present the palladium-catalyzed oxidative Heck reaction as a new and robust bio-orthogonal strategy for linking functionalized arylboronic acids to protein-bound alkenes in high yields a  ...[more]

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