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Cup-shaped superparamagnetic hemispheres for size-selective cell filtration.

ABSTRACT: We propose a new method of size separation of cells exploiting precisely size-controlled hemispherical superparamagnetic microparticles. A three-layered structure of a 2-nm nickel layer inserted between 15-nm silicon dioxide layers was formed on polystyrene cast spheres by vapor deposition. The polystyrene was then removed by burning and the hemispherical superparamagnetic microparticles, "magcups", were obtained. The standard target cells (CCRF-CEM, 12 ± 2??m) were mixed with a set of different sizes of the fabricated magcups, and we confirmed that the cells were captured in the magcups having cavities larger than 15??m in diameter, and then gathered by magnetic force. The collected cells were grown in a culture medium without any damage. The results suggest that this method is quick, simple and non-invasive size separation of target cells.

SUBMITTER: Kim H

PROVIDER: S-EPMC4163672 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature

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Cup-shaped superparamagnetic hemispheres for size-selective cell filtration.

Kim Hyonchol H Terazono Hideyuki H Takei Hiroyuki H Yasuda Kenji K

Scientific reports 20140915

We propose a new method of size separation of cells exploiting precisely size-controlled hemispherical superparamagnetic microparticles. A three-layered structure of a 2-nm nickel layer inserted between 15-nm silicon dioxide layers was formed on polystyrene cast spheres by vapor deposition. The polystyrene was then removed by burning and the hemispherical superparamagnetic microparticles, "magcups", were obtained. The standard target cells (CCRF-CEM, 12 ± 2 μm) were mixed with a set of different ...[more]

PMID: 25219418

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Insights from ANA-grade angiosperms into the early evolution of CUP-SHAPED COTYLEDON genes.

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