A universal approach to prepare reagents for DNA-assisted protein analysis.
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ABSTRACT:
SUBMITTER: Yan J
PROVIDER: S-EPMC4169473 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Publications
A universal approach to prepare reagents for DNA-assisted protein analysis.
Yan Junhong J Gu Gucci Jijuan GJ Jost Christian C Hammond Maria M Plückthun Andreas A Landegren Ulf U Kamali-Moghaddam Masood M
PloS one 20140918 9
The quality of DNA-labeled affinity probes is critical in DNA-assisted protein analyses, such as proximity ligation and extension assays, immuno-PCR, and immuno-rolling circle amplification reactions. Efficient, high-performance methods are therefore required for isolation of pure conjugates from reactions where DNA strands have been coupled to antibodies or recombinant affinity reagents. Here we describe a universal, scalable approach for preparing high-quality oligonucleotide-protein conjugate ...[more]
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