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A universal DNA-based protein detection system.

ABSTRACT: Protein immune detection requires secondary antibodies which must be carefully selected in order to avoid interspecies cross-reactivity, and is therefore restricted by the limited availability of primary/secondary antibody pairs. Here we present a versatile DNA-based protein detection system using a universal adapter to interface between IgG antibodies and DNA-modified reporter molecules. As a demonstration of this capability, we successfully used DNA nano-barcodes, quantum dots, and horseradish peroxidase enzyme to detect multiple proteins using our DNA-based labeling system. Our system not only eliminates secondary antibodies but also serves as a novel method platform for protein detection with modularity, high capacity, and multiplexed capability.

SUBMITTER: Tran TN

PROVIDER: S-EPMC4237005 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature

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A universal DNA-based protein detection system.

Tran Thua N N TN Cui Jinhui J Hartman Mark R MR Peng Songming S Funabashi Hisakage H Duan Faping F Yang Dayong D March John C JC Lis John T JT Cui Haixin H Luo Dan D

Journal of the American Chemical Society 20130911 38

Protein immune detection requires secondary antibodies which must be carefully selected in order to avoid interspecies cross-reactivity, and is therefore restricted by the limited availability of primary/secondary antibody pairs. Here we present a versatile DNA-based protein detection system using a universal adapter to interface between IgG antibodies and DNA-modified reporter molecules. As a demonstration of this capability, we successfully used DNA nano-barcodes, quantum dots, and horseradish ...[more]

PMID: 23978265

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