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A major deletion in the V?-J? intervening region results in hyperelevated transcription of proximal V? genes and a severely restricted repertoire.

ABSTRACT: Our previous studies have shown that DNase I hypersensitive sites 1 and 2 (HS1-2) and HS3-6 within the mouse V?-J? intervening region are essential for controlling locus contraction and creating a diverse Ab repertoire. In this article, we demonstrate that a 6.3-kb deletion encompassing HS1-6 altogether not only leads to the predictable sums of these phenotypes, but also results in a novel hyperelevation of transcription of proximal V? genes, in both pre-B and splenic B cells. These findings reveal previously unrecognized additional functions for cis-elements within the V?-J? intervening region, namely, prevention of the production of massive levels of noncoding RNA species by silencing transcription of germline proximal V? genes in both developing and mature B cells.

SUBMITTER: Xiang Y

PROVIDER: S-EPMC4170056 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature

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A major deletion in the Vκ-Jκ intervening region results in hyperelevated transcription of proximal Vκ genes and a severely restricted repertoire.

Xiang Yougui Y Park Sung-Kyun SK Garrard William T WT

Journal of immunology (Baltimore, Md. : 1950) 20140903 7

Our previous studies have shown that DNase I hypersensitive sites 1 and 2 (HS1-2) and HS3-6 within the mouse Vκ-Jκ intervening region are essential for controlling locus contraction and creating a diverse Ab repertoire. In this article, we demonstrate that a 6.3-kb deletion encompassing HS1-6 altogether not only leads to the predictable sums of these phenotypes, but also results in a novel hyperelevation of transcription of proximal Vκ genes, in both pre-B and splenic B cells. These findings rev ...[more]

PMID: 25187654

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