An optimized optogenetic clustering tool for probing protein interaction and function.
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ABSTRACT:
SUBMITTER: Taslimi A
PROVIDER: S-EPMC4170572 | biostudies-literature | 2014 Sep
REPOSITORIES: biostudies-literature
Publications
An optimized optogenetic clustering tool for probing protein interaction and function.
Taslimi Amir A Vrana Justin D JD Chen Daniel D Borinskaya Sofya S Mayer Bruce J BJ Kennedy Matthew J MJ Tucker Chandra L CL
Nature communications 20140918
The Arabidopsis photoreceptor cryptochrome 2 (CRY2) was previously used as an optogenetic module, allowing spatiotemporal control of cellular processes with light. Here we report the development of a new CRY2-derived optogenetic module, 'CRY2olig', which induces rapid, robust, and reversible protein oligomerization in response to light. Using this module, we developed a novel protein interaction assay, Light-Induced Co-clustering, that can be used to interrogate protein interaction dynamics in l ...[more]
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