Ontology highlight
ABSTRACT:
SUBMITTER: Jost AP
PROVIDER: S-EPMC4609243 | biostudies-literature | 2015 Oct
REPOSITORIES: biostudies-literature
Jost Anna Payne-Tobin AP Weiner Orion D OD
ACS synthetic biology 20150602 10
We recently developed a technique for rapidly and reversibly inhibiting protein function through light-inducible sequestration of proteins away from their normal sites of action. Here, we adapt this method for inducible inactivation of Bem1, a scaffold protein involved in budding yeast polarity. We find that acute inhibition of Bem1 produces profound defects in cell polarization and cell viability that are not observed in bem1Δ. By disrupting Bem1 activity at specific points in the cell cycle, w ...[more]