Dataset Information
Neurogenic differentiation of human dental stem cells in vitro.
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ABSTRACT: Objectives
The purpose of this study was to investigate the neurogenic differentiation of human dental pulp stem cells (DPSCs), periodontal ligament stem cells (PDLSCs), and stem cells from apical papilla (SCAP).Materials and methods
After induction of neurogenic differentiation using commercial differentiation medium, expression levels of neural markers, microtubule-associated protein 2 (MAP2), class III ?-tubulin, and glial fibrillary acidic protein (GFAP) were identified using reverse transcriptase polymerase chain reaction (PCR), real-time PCR, and immunocytochemistry.Results
The induced cells showed neuron-like morphologies, similar to axons, dendrites, and perikaryons, which are composed of neurons in DPSCs, PDLSCs, and SCAP. The mRNA levels of neuronal markers tended to increase in differentiated cells. The expression of MAP2 and ?-tubulin III also increased at the protein level in differentiation groups, even though GFAP was not detected via immunocytochemistry.Conclusion
Human dental stem cells including DPSCs, PDLSCs, and SCAP may have neurogenic differentiation capability in vitro. The presented data support the use of human dental stem cells as a possible alternative source of stem cells for therapeutic utility in the treatment of neurological diseases.
SUBMITTER: Lee JH
PROVIDER: S-EPMC4170666 | biostudies-literature | 2014 Aug
REPOSITORIES: biostudies-literature
Publications
Neurogenic differentiation of human dental stem cells in vitro.
Journal of the Korean Association of Oral and Maxillofacial Surgeons 20140826 4
<h4>Objectives</h4>The purpose of this study was to investigate the neurogenic differentiation of human dental pulp stem cells (DPSCs), periodontal ligament stem cells (PDLSCs), and stem cells from apical papilla (SCAP).<h4>Materials and methods</h4>After induction of neurogenic differentiation using commercial differentiation medium, expression levels of neural markers, microtubule-associated protein 2 (MAP2), class III β-tubulin, and glial fibrillary acidic protein (GFAP) were identified using ...[more]