Dataset Information

Lidocaine inhibits osteogenic differentiation of human dental pulp stem cells in vitro.

ABSTRACT:

Objective

Lidocaine is an amide local anaesthetic commonly used for pain control, however, few studies have investigated the effect of lidocaine on the osteogenic differentiation of human dental pulp stem cells (HDPSCs). The present study aimed to determine the effect of lidocaine on HDPSC viability and osteogenic differentiation.

Methods

HDPSCs were incubated with 0, 0.05, 0.2, 0.5, and 1 mM lidocaine for 24, 48 and 72 h, after which, MTT assays were performed. HDPSCs cultured with the above lidocaine concentrations and osteogenic differentiation medium for 7 and 14 days were stained for alkaline phosphatase (ALP). Protein and mRNA levels of relevant osteogenic factors (bone morphogenetic protein-2 [BMP-2] and runt-related transcription factor 2 [RUNX2]) were examined using western blotting and real-time reverse-transcription polymerase chain reaction, respectively.

Results

Lidocaine did not affect the viability of HDPSCs, however, lidocaine reduced ALP activity in HDPSCs. Levels of ALP, BMP-2, and RUNX2 mRNA were reduced with lidocaine, and levels of BMP-2 and RUNX2 proteins were decreased, versus controls.

Conclusions

Lidocaine inhibits osteogenic differentiation markers in HDPSCs in vitro, even at low concentrations, without cytotoxicity. This study suggests that lidocaine may inhibit osteogenic differentiation in HDPSC-mediated regenerative medicine, including pulp regeneration and repair.

SUBMITTER: Kim EJ

PROVIDER: S-EPMC9909061 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature

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Publications

Lidocaine inhibits osteogenic differentiation of human dental pulp stem cells <i>in vitro</i>.

Kim Eun-Jung EJ Yoon Ji-Uk JU Kim Cheul-Hong CH Yoon Ji-Young JY Kim Joo-Young JY Kim Hyang-Sook HS Choi Eun-Ji EJ

The Journal of international medical research 20230201 2

<h4>Objective</h4>Lidocaine is an amide local anaesthetic commonly used for pain control, however, few studies have investigated the effect of lidocaine on the osteogenic differentiation of human dental pulp stem cells (HDPSCs). The present study aimed to determine the effect of lidocaine on HDPSC viability and osteogenic differentiation.<h4>Methods</h4>HDPSCs were incubated with 0, 0.05, 0.2, 0.5, and 1 mM lidocaine for 24, 48 and 72 h, after which, MTT assays were performed. HDPSCs cultured wi ...[more]

PMID: 36748349

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