Ontology highlight
ABSTRACT:
SUBMITTER: Podesta A
PROVIDER: S-EPMC4171011 | biostudies-literature | 2014 Jul-Aug
REPOSITORIES: biostudies-literature
Podestà Adriano A Rossi Serena S Massarelli Ilaria I Carpi Sara S Adinolfi Barbara B Fogli Stefano S Bianucci Anna Maria AM Nieri Paola P
mAbs 20140701 4
Organophosphates are potent poisoning agents that cause severe cholinergic toxicity. Current treatment has been reported to be unsatisfactory and novel antidotes are needed. In this study, we used a single-chain variable fragment (scFv) library to select a recombinant antibody fragment (WZ1-14.2.1) with butyrylcholinesterase-like catalytic activity by using an innovative method integrating genetic selection and the bait-and-switch strategy. Ellman assay demonstrated that WZ1-14.2.1 has Michaelis ...[more]