Ontology highlight
ABSTRACT:
SUBMITTER: Ranaei-Siadat E
PROVIDER: S-EPMC4174442 | biostudies-literature | 2014 Oct
REPOSITORIES: biostudies-literature
Ranaei-Siadat Ehsan E Mérigoux Cécile C Seijo Bili B Ponchon Luc L Saliou Jean-Michel JM Bernauer Julie J Sanglier-Cianférani Sarah S Dardel Fréderic F Vachette Patrice P Nonin-Lecomte Sylvie S
RNA (New York, N.Y.) 20140818 10
TmRNA is an abundant RNA in bacteria with tRNA and mRNA features. It is specialized in trans-translation, a translation rescuing system. We demonstrate that its partner protein SmpB binds the tRNA-like region (TLD) in vivo and chaperones the fold of the TLD-H2 region. We use an original approach combining the observation of tmRNA degradation pathways in a heterologous system, the analysis of the tmRNA digests by MS and NMR, and co-overproduction assays of tmRNA and SmpB. We study the conformatio ...[more]