A novel polymorphism of the GP78 gene is associated with coronary artery disease in Han population in China.

ABSTRACT: GP78 is a membrane-anchored ubiquitin ligase mediating the degradation of 3-hydroxy-3-methyl-glutaryl-CoA coenzyme A reductase (HMGCR) and Insig-1, which was very essential for the synthesis of cholesterol process. Cholesterol levels have a causal role in the development of cardiovascular disease. The aim of the present study was to assess the association between the human gp78 gene polymorphism and coronary artery disease (CAD) in a Han and Uygur population of China.We used two independent case-control studies: a Han population (602 CAD patients and 572 control subjects) and a Uygur population (374 CAD patients and 376 control subjects). All CAD patients and controls were genotyped for the same three single nucleotide polymorphisms (SNPs) (rs731119, rs2617849 and rs2440472) of gp78 gene by a Real-time PCR instrument.In the Han population, for total and men, the distribution of SNP3 (rs2440472) alleles and the dominant model (AA vs AG?+?GG) and recessive model (GG vs AG?+?AA) showed a significant difference between CAD and control participants (for allele: P?=?0.003 and P?=?0.002, respectively; for dominant model: P?=?0.041 and P?=?0.026, respectively; for recessive model: p?=?0.004 and p?=?0.004, respectively).The significant difference in both the two models was retained after adjustment for covariates (for dominant model OR:0.760, 95% confidence interval [CI]:0.584-0.99, P?=?0.042; OR:0.686, 95% CI: 0.498-0.946, P?=?0.022, respectively; for recessive model OR: 1.451, 95% CI: 1.067-1.974, P?=?0.018; OR: 1.789, 95% CI: 1.219-2.627, P?=?0.000). Our data was also assessed via haplotype-based case-control studies. For the Han population, for total, The G-T-G haplotype in CAD was significantly higher than that in the control group (P?=?0.02), and the G-C-A haplotype in CAD was significantly lower than that in the control group (P?=?0.0443), And for man, the G-T-G haplotype in CAD was significantly higher than that in the control group (P?=?0.0048).The GG genotype and G allele of rs2440472 in gp78 gene could be a risk genetic marker of CAD in Han population in China.

SUBMITTER: Cha E 

PROVIDER: S-EPMC4175632 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature