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Labeled protein recognition at a membrane bilayer interface by embedded synthetic receptors.

ABSTRACT: Self-folding deep cavitands embedded in a supported lipid bilayer are capable of recognizing suitably labeled proteins at the bilayer interface. The addition of a choline derived binding "handle" to a number of different proteins allows their selective noncovalent recognition, with association constants on the order of 10(5) M(-1). The proteins are displayed at the water:bilayer interface, and a single binding handle allows recognition of the large, charged protein by a small molecule synthetic receptor via complementary shape and charge interactions.

SUBMITTER: Ghang YJ

PROVIDER: S-EPMC4176395 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature

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Labeled protein recognition at a membrane bilayer interface by embedded synthetic receptors.

Ghang Yoo-Jin YJ Lloyd Jonathan J JJ Moehlig Melissa P MP Arguelles Jessica K JK Mettry Magi M Zhang Xing X Julian Ryan R RR Cheng Quan Q Hooley Richard J RJ

Langmuir : the ACS journal of surfaces and colloids 20140819 34

Self-folding deep cavitands embedded in a supported lipid bilayer are capable of recognizing suitably labeled proteins at the bilayer interface. The addition of a choline derived binding "handle" to a number of different proteins allows their selective noncovalent recognition, with association constants on the order of 10(5) M(-1). The proteins are displayed at the water:bilayer interface, and a single binding handle allows recognition of the large, charged protein by a small molecule synthetic ...[more]

PMID: 25130415

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