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Digital microfluidic processing of mammalian embryos for vitrification.

ABSTRACT: Cryopreservation is a key technology in biology and clinical practice. This paper presents a digital microfluidic device that automates sample preparation for mammalian embryo vitrification. Individual micro droplets manipulated on the microfluidic device were used as micro-vessels to transport a single mouse embryo through a complete vitrification procedure. Advantages of this approach, compared to manual operation and channel-based microfluidic vitrification, include automated operation, cryoprotectant concentration gradient generation, and feasibility of loading and retrieval of embryos.

SUBMITTER: Pyne DG

PROVIDER: S-EPMC4176959 | biostudies-literature |

REPOSITORIES: biostudies-literature

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