The linker region of NS3 plays a critical role in the replication and infectivity of hepatitis C virus.
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ABSTRACT:
SUBMITTER: Kohlway A
PROVIDER: S-EPMC4178846 | biostudies-literature | 2014 Sep
REPOSITORIES: biostudies-literature
Publications
The linker region of NS3 plays a critical role in the replication and infectivity of hepatitis C virus.
Kohlway Andrew A Pirakitikulr Nathan N Ding Steve C SC Yang Feng F Luo Dahai D Lindenbach Brett D BD Pyle Anna M AM
Journal of virology 20140625 18
Hepatitis C virus (HCV) NS3-4A is required for viral replication and assembly. We establish that virus assembly is sensitive to mutations in the linker region between the helicase and protease domains of NS3-4A. However, we find that the protease cleavage, RNA binding, and unwinding rates of NS3 are minimally affected in vitro. Thus, we conclude that the NS3 linker is critical for mediating protein-protein interactions and dynamic control rather than for modulating the enzymatic functions of NS3 ...[more]
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