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The linker region of NS3 plays a critical role in the replication and infectivity of hepatitis C virus.


ABSTRACT: Hepatitis C virus (HCV) NS3-4A is required for viral replication and assembly. We establish that virus assembly is sensitive to mutations in the linker region between the helicase and protease domains of NS3-4A. However, we find that the protease cleavage, RNA binding, and unwinding rates of NS3 are minimally affected in vitro. Thus, we conclude that the NS3 linker is critical for mediating protein-protein interactions and dynamic control rather than for modulating the enzymatic functions of NS3-4A.

SUBMITTER: Kohlway A 

PROVIDER: S-EPMC4178846 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature

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The linker region of NS3 plays a critical role in the replication and infectivity of hepatitis C virus.

Kohlway Andrew A   Pirakitikulr Nathan N   Ding Steve C SC   Yang Feng F   Luo Dahai D   Lindenbach Brett D BD   Pyle Anna M AM  

Journal of virology 20140625 18


Hepatitis C virus (HCV) NS3-4A is required for viral replication and assembly. We establish that virus assembly is sensitive to mutations in the linker region between the helicase and protease domains of NS3-4A. However, we find that the protease cleavage, RNA binding, and unwinding rates of NS3 are minimally affected in vitro. Thus, we conclude that the NS3 linker is critical for mediating protein-protein interactions and dynamic control rather than for modulating the enzymatic functions of NS3  ...[more]

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