Dataset Information

Whole-genome haplotype reconstruction using proximity-ligation and shotgun sequencing.

ABSTRACT: Rapid advances in high-throughput sequencing facilitate variant discovery and genotyping, but linking variants into a single haplotype remains challenging. Here we demonstrate HaploSeq, an approach for assembling chromosome-scale haplotypes by exploiting the existence of 'chromosome territories'. We use proximity ligation and sequencing to show that alleles on homologous chromosomes occupy distinct territories, and therefore this experimental protocol preferentially recovers physically linked DNA variants on a homolog. Computational analysis of such data sets allows for accurate (?99.5%) reconstruction of chromosome-spanning haplotypes for ?95% of alleles in hybrid mouse cells with 30× sequencing coverage. To resolve haplotypes for a human genome, which has a low density of variants, we coupled HaploSeq with local conditional phasing to obtain haplotypes for ?81% of alleles with ?98% accuracy from just 17× sequencing. Whereas methods based on proximity ligation were originally designed to investigate spatial organization of genomes, our results lend support for their use as a general tool for haplotyping.

SUBMITTER: Selvaraj S

PROVIDER: S-EPMC4180835 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature

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Publications

Whole-genome haplotype reconstruction using proximity-ligation and shotgun sequencing.

Selvaraj Siddarth S R Dixon Jesse J Bansal Vikas V Ren Bing B

Nature biotechnology 20131103 12

Rapid advances in high-throughput sequencing facilitate variant discovery and genotyping, but linking variants into a single haplotype remains challenging. Here we demonstrate HaploSeq, an approach for assembling chromosome-scale haplotypes by exploiting the existence of 'chromosome territories'. We use proximity ligation and sequencing to show that alleles on homologous chromosomes occupy distinct territories, and therefore this experimental protocol preferentially recovers physically linked DN ...[more]

PMID: 24185094

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Dataset Information

Whole-genome haplotype reconstruction using proximity-ligation and shotgun sequencing.

Publications

Whole-genome haplotype reconstruction using proximity-ligation and shotgun sequencing.

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