Ontology highlight
ABSTRACT:
SUBMITTER: Tucker CL
PROVIDER: S-EPMC4181358 | biostudies-literature | 2014 Sep
REPOSITORIES: biostudies-literature
Tucker Chandra L CL Vrana Justin D JD Kennedy Matthew J MJ
Current protocols in cell biology 20140902
Genetically encoded actuators that allow control of protein-protein interactions using light, termed 'optical dimerizers', are emerging as new tools for experimental biology. In recent years, numerous new and versatile dimerizer systems have been developed. Here we discuss the design of optical dimerizer experiments, including choice of a dimerizer system, photoexcitation sources, and the coordinate use of imaging reporters. We provide detailed protocols for experiments using two dimerization sy ...[more]