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Infection dynamics of sylvatic dengue virus in a natural primate host, the African Green Monkey.

ABSTRACT: The four serotypes of mosquito-borne dengue virus (DENV-1, -2, -3, and -4) that circulate in humans each emerged from an enzootic, sylvatic cycle in non-human primates. Herein, we present the first study of sylvatic DENV infection dynamics in a primate. Three African green monkeys were inoculated with 10(5) plaque-forming units (pfu) DENV-2 strain PM33974 from the sylvatic cycle, and one African green monkey was inoculated with 10(5) pfu DENV-2 strain New Guinea C from the human cycle. All four monkeys seroconverted (more than fourfold rise in 80% plaque reduction neutralization titer [PRNT80]) against the strain of DENV with which they were inoculated; only one (33%) of three monkeys infected with sylvatic DENV showed a neutralizing antibody response against human-endemic DENV. Virus was detected in two of three monkeys inoculated with sylvatic DENV at low titer (? 1.3 log10pfu/mL) and brief duration (? 2 days). Clinical signs included rash and elevated aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels.

SUBMITTER: Hanley KA

PROVIDER: S-EPMC4183386 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature

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Infection dynamics of sylvatic dengue virus in a natural primate host, the African Green Monkey.

Hanley Kathryn A KA Guerbois Mathilde M Kautz Tiffany F TF Brown Meredith M Whitehead Stephen S SS Weaver Scott C SC Vasilakis Nikos N Marx Preston A PA

The American journal of tropical medicine and hygiene 20140804 4

The four serotypes of mosquito-borne dengue virus (DENV-1, -2, -3, and -4) that circulate in humans each emerged from an enzootic, sylvatic cycle in non-human primates. Herein, we present the first study of sylvatic DENV infection dynamics in a primate. Three African green monkeys were inoculated with 10(5) plaque-forming units (pfu) DENV-2 strain PM33974 from the sylvatic cycle, and one African green monkey was inoculated with 10(5) pfu DENV-2 strain New Guinea C from the human cycle. All four ...[more]

PMID: 25092823

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Dataset Information

Infection dynamics of sylvatic dengue virus in a natural primate host, the African Green Monkey.

Publications

Infection dynamics of sylvatic dengue virus in a natural primate host, the African Green Monkey.

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