Ontology highlight
ABSTRACT:
SUBMITTER: Bitsikas V
PROVIDER: S-EPMC4185422 | biostudies-literature | 2014 Sep
REPOSITORIES: biostudies-literature
Bitsikas Vassilis V Corrêa Ivan R IR Nichols Benjamin J BJ
eLife 20140917
Several different endocytic pathways have been proposed to function in mammalian cells. Clathrin-coated pits are well defined, but the identity, mechanism and function of alternative pathways have been controversial. Here we apply universal chemical labelling of plasma membrane proteins to define all primary endocytic vesicles, and labelling of specific proteins with a reducible SNAP-tag substrate. These approaches provide high temporal resolution and stringent discrimination between surface-con ...[more]