Ontology highlight
ABSTRACT:
SUBMITTER: Kumar S
PROVIDER: S-EPMC4188501 | biostudies-literature | 2014 Jun
REPOSITORIES: biostudies-literature
Indian journal of microbiology 20131116 2
A rapid and sensitive two-step RT-PCR protocol for simultaneous detection of major apple viruses, namely Apple mosaic virus (ApMV), Apple stem pitting virus (ASPV), Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV) and Apple scar skin viroid (ASSVd), was developed. Five specific primer pairs were tested and confirmed for these viruses and viroid together in a single tube, giving amplicons of ~198, ~330, ~370, ~547 and ~645 bp corresponding to ASGV, ASSVd, ASPV, ApMV and A ...[more]