Ontology highlight
ABSTRACT:
SUBMITTER: Malandraki I
PROVIDER: S-EPMC5547701 | biostudies-literature | 2017
REPOSITORIES: biostudies-literature
Malandraki Ioanna I Beris Despoina D Isaioglou Ioannis I Olmos Antonio A Varveri Christina C Vassilakos Nikon N
PloS one 20170727 7
A one-step multiplex real-time reverse transcription polymerase chain reaction (RT-qPCR) based on TaqMan probes was developed for the simultaneous detection of Apple mosaic virus (ApMV), Apple stem pitting virus (ASPV) and Apple stem grooving virus (ASGV) in total RNA of pome trees extracted with a CTAB method. The sensitivity of the method was established using in vitro synthesized viral transcripts serially diluted in RNA from healthy, virus-tested (negative) pome trees. The three viruses were ...[more]