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Reversible positioning of single molecules inside zero-mode waveguides.

ABSTRACT: We have developed a hybrid nanopore/zero-mode waveguide device for single-molecule fluorescence and DNA sequencing applications. The device is a freestanding solid-state membrane with sub-5 nm nanopores that reversibly delivers individual biomolecules to the base of 70 nm diameter waveguides for interrogation. Rapid and reversible molecular loading is achieved by controlling the voltage across the device. Using this device we demonstrate protein and DNA loading with efficiency that is orders of magnitude higher than diffusion-based molecular loading.

SUBMITTER: Larkin J 

PROVIDER: S-EPMC4189617 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature

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Reversible positioning of single molecules inside zero-mode waveguides.

Larkin Joseph J   Foquet Mathieu M   Turner Stephen W SW   Korlach Jonas J   Wanunu Meni M  

Nano letters 20140915 10

We have developed a hybrid nanopore/zero-mode waveguide device for single-molecule fluorescence and DNA sequencing applications. The device is a freestanding solid-state membrane with sub-5 nm nanopores that reversibly delivers individual biomolecules to the base of 70 nm diameter waveguides for interrogation. Rapid and reversible molecular loading is achieved by controlling the voltage across the device. Using this device we demonstrate protein and DNA loading with efficiency that is orders of  ...[more]

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