Ontology highlight
ABSTRACT:
SUBMITTER: Liu Y
PROVIDER: S-EPMC4189618 | biostudies-literature | 2014 Oct
REPOSITORIES: biostudies-literature
Liu Yang Y Medda Rebecca R Liu Zheng Z Galior Kornelia K Yehl Kevin K Spatz Joachim P JP Cavalcanti-Adam Elisabetta Ada EA Salaita Khalid K
Nano letters 20140930 10
Herein we aimed to understand how nanoscale clustering of RGD ligands alters the mechano-regulation of their integrin receptors. We combined molecular tension fluorescence microscopy with block copolymer micelle nanolithography to fabricate substrates with arrays of precisely spaced probes that can generate a 10-fold fluorescence response to pN-forces. We found that the mechanism of sensing ligand spacing is force-mediated. This strategy is broadly applicable to investigating receptor clustering ...[more]