Ontology highlight
ABSTRACT:
SUBMITTER: Zhao Y
PROVIDER: S-EPMC6613363 | biostudies-literature | 2019 Apr
REPOSITORIES: biostudies-literature
Zhao Yuanchang Y Wetter Nathaniel M NM Wang Xuefeng X
Journal of visualized experiments : JoVE 20190425 146
Molecular tension transmitted by integrin-ligand bonds is the fundamental mechanical signal in the integrin pathway that plays significant roles in many cell functions and behaviors. To calibrate and image integrin tension with high force sensitivity and spatial resolution, we developed an integrative tension sensor (ITS), a DNA-based fluorescent tension sensor. The ITS is activated to fluoresce if sustaining a molecular tension, thus converting force to fluorescent signal at the molecular level ...[more]