Ontology highlight
ABSTRACT:
SUBMITTER: Maier CS
PROVIDER: S-EPMC4191911 | biostudies-literature | 2010
REPOSITORIES: biostudies-literature
Maier Claudia S CS Chavez Juan J Wang Jing J Wu Jianyong J
Methods in enzymology 20100101
This chapter describes a mass spectrometry-based strategy that facilitates the unambiguous identification and characterization of proteins modified by lipid peroxidation-derived 2-alkenals. The approach employs a biotinylated hydroxyl amine derivative as an aldehyde/keto-reactive probe in conjunction with selective enrichment and tandem mass spectrometric analysis. Methodological details are given for model studies involving a distinct protein and 4-hydroxy-2-nonenal (HNE). The method was also e ...[more]