Ontology highlight
ABSTRACT:
SUBMITTER: Osipovich AB
PROVIDER: S-EPMC419606 | biostudies-literature | 2004
REPOSITORIES: biostudies-literature
Osipovich Anna B AB White-Grindley Erica K EK Hicks Geoffrey G GG Roshon Michael J MJ Shaffer Christian C Moore Jason H JH Ruley H Earl HE
Nucleic acids research 20040520 9
Gene trap vectors developed for genome-wide mutagenesis can be used to study factors governing the expression of exons inserted throughout the genome. For example, entrapment vectors consisting of a partial 3'-terminal exon [i.e. a neomycin resistance gene (Neo), a poly(A) site, but no 3' splice site] were typically expressed following insertion into introns, from cellular transcripts that spliced to cryptic 3' splice sites present either within the targeting vector or in the adjacent intron. A ...[more]