Ontology highlight
ABSTRACT:
SUBMITTER: Zhegalova NG
PROVIDER: S-EPMC4198576 | biostudies-literature | 2014 Sep-Oct
REPOSITORIES: biostudies-literature
Zhegalova Natalia G NG He Shawn S Zhou Haiying H Kim David M DM Berezin Mikhail Y MY
Contrast media & molecular imaging 20140425 5
Self-aggregation of dyes even at low concentrations poses a considerable challenge in preparing sufficiently bright molecular probes for in vivo imaging, particularly in the conjugation of near infrared cyanine dyes to polypeptides with multiple labeling sites. Such self-aggregation leads to a significant energy transfer between the dyes, resulting in severe quenching and low brightness of the targeted probe. To address this problem, we designed a novel type of dye with an asymmetrical distribut ...[more]