Ontology highlight
ABSTRACT:
SUBMITTER: Van Deventer JA
PROVIDER: S-EPMC4199383 | biostudies-literature | 2014 Aug
REPOSITORIES: biostudies-literature
Chembiochem : a European journal of chemical biology 20140717 12
Non-canonical amino acids (ncAAs) provide powerful tools for engineering the chemical and physical properties of proteins. However, introducing ncAAs into proteins can affect protein properties in unpredictable ways, thus necessitating screening efforts to identify mutants with desirable properties. In this work, we describe an Escherichia coli cell surface display platform for the directed evolution of clickable antibody fragments. This platform enabled isolation of antibody fragments with impr ...[more]