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Acetylation-defective mutant of Ppar? is associated with decreased lipid synthesis in breast cancer cells.


ABSTRACT: In our prior publications we characterized a conserved acetylation motif (K(R)xxKK) of evolutionarily related nuclear receptors. Recent reports showed that peroxisome proliferator activated receptor gamma (PPAR?) deacetylation by SIRT1 is involved in delaying cellular senescence and maintaining the brown remodeling of white adipose tissue. However, it still remains unknown whether lysyl residues 154 and 155 (K154/155) of the conserved acetylation motif (RIHKK) in Ppar?1 are acetylated. Herein, we demonstrate that Ppar?1 is acetylated and regulated by both endogenous TSA-sensitive and NAD-dependent deacetylases. Acetylation of lysine 154 was identified by mass spectrometry (MS) while deacetylation of lysine 155 by SIRT1 was confirmed by in vitro deacetylation assay. An in vivo labeling assay revealed K154/K155 as bona fide acetylation sites. The conserved acetylation sites of Ppar?1 and the catalytic domain of SIRT1 are both required for the interaction between Ppar?1 and SIRT1. Sirt1 and Ppar?1 converge to govern lipid metabolism in vivo. Acetylation-defective mutants of Ppar?1 were associated with reduced lipid synthesis in ErbB2 overexpressing breast cancer cells. Together, these results suggest that the conserved lysyl residues K154/K155 of Ppar?1 are acetylated and play an important role in lipid synthesis in ErbB2-positive breast cancer cells.

SUBMITTER: Tian L 

PROVIDER: S-EPMC4202124 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature

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Acetylation-defective mutant of Pparγ is associated with decreased lipid synthesis in breast cancer cells.

Tian Lifeng L   Wang Chenguang C   Hagen Fred K FK   Gormley Michael M   Addya Sankar S   Soccio Raymond R   Casimiro Mathew C MC   Zhou Jie J   Powell Michael J MJ   Xu Ping P   Deng Haiteng H   Sauve Anthony A AA   Pestell Richard G RG  

Oncotarget 20140901 17


In our prior publications we characterized a conserved acetylation motif (K(R)xxKK) of evolutionarily related nuclear receptors. Recent reports showed that peroxisome proliferator activated receptor gamma (PPARγ) deacetylation by SIRT1 is involved in delaying cellular senescence and maintaining the brown remodeling of white adipose tissue. However, it still remains unknown whether lysyl residues 154 and 155 (K154/155) of the conserved acetylation motif (RIHKK) in Pparγ1 are acetylated. Herein, w  ...[more]

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