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Divergent evolution of potato immune receptor CC domain interactions with the Ran GTPase-activating protein 2.

ABSTRACT: Effector-triggered immunity mediated by immune receptors in plants provides powerful defense against specific pathogens. Solanum tuberosum Ran GTPase-Activating Protein 2 (StRanGAP2) interacts with immune receptors Rx and Gpa2 through their coiled-coil (CC) domains. We assayed additional CC domains from other Solanaceous immune receptors and observed interaction by co-immunoprecipitation between StRanGAP2 and a novel immune receptor, STR5. A CC domain very similar to Rx and Gpa2, STR4, failed to interact, likely due to sequence divergence in the region implicated in StRanGAP2 binding. Like Rx and Gpa2, STR5 interacted with the StRanGAP2 N-terminal WPP domain. Our findings substantiate the importance of RanGAPs as common CC-interacting proteins of multiple immune receptors requiring further study to define their roles in pathogen perception.

SUBMITTER: Sobhanian S

PROVIDER: S-EPMC4203644 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Divergent evolution of potato immune receptor CC domain interactions with the Ran GTPase-activating protein 2.

Sobhanian Soha S Sacco Melanie M

Plant signaling & behavior 20140101 9

Effector-triggered immunity mediated by immune receptors in plants provides powerful defense against specific pathogens. Solanum tuberosum Ran GTPase-Activating Protein 2 (StRanGAP2) interacts with immune receptors Rx and Gpa2 through their coiled-coil (CC) domains. We assayed additional CC domains from other Solanaceous immune receptors and observed interaction by co-immunoprecipitation between StRanGAP2 and a novel immune receptor, STR5. A CC domain very similar to Rx and Gpa2, STR4, failed to ...[more]

PMID: 25763710

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Project description:Legionella pneumophila governs its interactions with host cells by secreting >300 different "effector" proteins. Some of these effectors contain eukaryotic domains such as the RCC1 (regulator of chromosome condensation 1) repeats promoting the activation of the small GTPase Ran. In this report, we reveal a conserved pattern of L. pneumophila RCC1 repeat genes, which are distributed in two main clusters of strains. Accordingly, strain Philadelphia-1 contains two RCC1 genes implicated in bacterial virulence, legG1 (Legionella eukaryotic gene 1), and ppgA, while strain Paris contains only one, pieG The RCC1 repeat effectors localize to different cellular compartments and bind distinct components of the Ran GTPase cycle, including Ran modulators and the small GTPase itself, and yet they all promote the activation of Ran. The pieG gene spans the corresponding open reading frames of legG1 and a separate adjacent upstream gene, lpg1975 legG1 and lpg1975 are fused upon addition of a single nucleotide to encode a protein that adopts the binding specificity of PieG. Thus, a point mutation in pieG splits the gene, altering the effector target. These results indicate that divergent evolution of RCC1 repeat effectors defines the Ran GTPase cycle targets and that modulation of different components of the cycle might fine-tune Ran activation during Legionella infection.IMPORTANCE Legionella pneumophila is a ubiquitous environmental bacterium which, upon inhalation, causes a life-threatening pneumonia termed Legionnaires' disease. The opportunistic pathogen grows in amoebae and macrophages by employing a "type IV" secretion system, which secretes more than 300 different "effector" proteins into the host cell, where they subvert pivotal processes. The function of many of these effector proteins is unknown, and their evolution has not been studied. L. pneumophila RCC1 repeat effectors target the small GTPase Ran, a molecular switch implicated in different cellular processes such as nucleocytoplasmic transport and microtubule cytoskeleton dynamics. We provide evidence that one or more RCC1 repeat genes are distributed in two main clusters of L. pneumophila strains and have divergently evolved to target different components of the Ran GTPase activation cycle at different subcellular sites. Thus, L. pneumophila employs a sophisticated strategy to subvert host cell Ran GTPase during infection.

Dataset Information

Divergent evolution of potato immune receptor CC domain interactions with the Ran GTPase-activating protein 2.

Publications

Divergent evolution of potato immune receptor CC domain interactions with the Ran GTPase-activating protein 2.

Similar Datasets

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