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Assessment of helminth biodiversity in wild rats using 18S rDNA based metagenomics.

ABSTRACT: Parasite diversity has important implications in several research fields including ecology, evolutionary biology and epidemiology. Wide-ranging analysis has been restricted because of the difficult, highly specialised and time-consuming processes involved in parasite identification. In this study, we assessed parasite diversity in wild rats using 18S rDNA-based metagenomics. 18S rDNA PCR products were sequenced using an Illumina MiSeq sequencer and the analysis of the sequences using the QIIME software successfully classified them into several parasite groups. The comparison of the results with those obtained using standard methods including microscopic observation of helminth parasites in the rat intestines and PCR amplification/sequencing of 18S rDNA from isolated single worms suggests that this new technique is reliable and useful to investigate parasite diversity.

SUBMITTER: Tanaka R

PROVIDER: S-EPMC4207705 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Assessment of helminth biodiversity in wild rats using 18S rDNA based metagenomics.

Tanaka Ryusei R Hino Akina A Tsai Isheng J IJ Palomares-Rius Juan Emilio JE Yoshida Ayako A Ogura Yoshitoshi Y Hayashi Tetsuya T Maruyama Haruhiko H Kikuchi Taisei T

PloS one 20141023 10

Parasite diversity has important implications in several research fields including ecology, evolutionary biology and epidemiology. Wide-ranging analysis has been restricted because of the difficult, highly specialised and time-consuming processes involved in parasite identification. In this study, we assessed parasite diversity in wild rats using 18S rDNA-based metagenomics. 18S rDNA PCR products were sequenced using an Illumina MiSeq sequencer and the analysis of the sequences using the QIIME s ...[more]

PMID: 25340824

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