Unknown

Dataset Information

0

MicroRNA signatures in chemotherapy resistant esophageal cancer cell lines.


ABSTRACT: To investigate expression of microRNA (miRNA) and potential targets in chemotherapy resistant esophageal cancer cell lines.An in-vitro model of acquired chemotherapy resistance in esophageal adeno- (EAC) and squamous cell carcinoma (ESCC) cells was used, and microRNA expression profiles for cisplatin or 5-fluorouracil (5-FU) resistant variants vs chemotherapy sensitive controls were compared using microarray and quantitative real-time polymerase chain reaction (PCR). The expression of chemotherapy-relevant genes potentially targeted by the dysregulated microRNAs in the chemotherapy resistant variants was also evaluated.Chemotherapy resistant sublines were found to have specific miRNA signatures, and these miRNA signatures were different for the cisplatin vs 5-FU resistant cells from the same tumor cell line, and also for EAC vs ESCC cells with resistance to the same specific chemotherapy agent. Amongst others, miR-27b-3p, miR-193b-3p, miR-192-5p, miR-378 a-3p, miR-125a-5p and miR-18a-3p were dysregulated, consistent with negative posttranscriptional control of KRAS, TYMS, ABCC3, CBL-B and ERBB2 expression via these miRNAs.The current study supports the hypothesis that microRNA expression has an impact on chemotherapy resistance in esophageal cancer.

SUBMITTER: Hummel R 

PROVIDER: S-EPMC4209553 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

MicroRNA signatures in chemotherapy resistant esophageal cancer cell lines.

Hummel Richard R   Sie Corina C   Watson David I DI   Wang Tingting T   Ansar Alfiya A   Michael Michael Z MZ   Van der Hoek Mark M   Haier Joerg J   Hussey Damian J DJ  

World journal of gastroenterology 20141001 40


<h4>Aim</h4>To investigate expression of microRNA (miRNA) and potential targets in chemotherapy resistant esophageal cancer cell lines.<h4>Methods</h4>An in-vitro model of acquired chemotherapy resistance in esophageal adeno- (EAC) and squamous cell carcinoma (ESCC) cells was used, and microRNA expression profiles for cisplatin or 5-fluorouracil (5-FU) resistant variants vs chemotherapy sensitive controls were compared using microarray and quantitative real-time polymerase chain reaction (PCR).  ...[more]

Similar Datasets

2013-08-28 | GSE50224 | GEO
2013-08-28 | E-GEOD-50224 | biostudies-arrayexpress
| S-EPMC9307586 | biostudies-literature
| S-ECPF-GEOD-17775 | biostudies-other
| S-EPMC3205057 | biostudies-literature
| S-EPMC5103481 | biostudies-literature
| S-EPMC5993214 | biostudies-literature
| S-EPMC3445463 | biostudies-literature
| S-ECPF-GEOD-52802 | biostudies-other
| S-EPMC2745487 | biostudies-literature