Project description:Extracellular vesicles (EVs) are increasingly recognized as key mediators of intercellular communication and immune regulation. However, their proteomic composition in allergic asthma remains poorly understood. In this study, we performed tandem mass tag (TMT)-based quantitative proteomic analyses of the whole bronchoalveolar lavage fluid (BALF) supernatant and EVs enriched from BALF in a murine model of house dust mite (HDM)-induced asthma. Using LC-MS/MS analysis in the data-dependent acquisition (DDA) mode, we identified more than 400 proteins across all samples, revealing distinct enrichment of canonical EV markers in EV fractions. HDM challenge induced significant upregulation of Th2-associated proteins, e.g., CLCA1, FCGBP, CHIL3, CHIL4, and RETNLA, consistent with hallmark features of asthma. Notably, proteins such as EPX and CKM were detected exclusively in EVs, not in whole BALF, underscoring the potential of EVs to selectively carry disease-relevant proteins. Our results provided insights into the distinct proteomic profiles of EV-associated versus BALF proteins, highlighting potential EV-mediated mechanisms in asthma pathogenesis.

2026-04-20 | PXD073977 | Pride

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