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Synthesis and antibacterial activities of antibacterial peptides with a spiropyran fluorescence probe.

ABSTRACT: In this report, antibacterial peptides 1-3 were prepared with a spiropyran fluorescence probe. The probe exhibits a change in fluorescence when isomerized from a colorless spiro-form (spiropyran, Sp) to a colored open-form (merocyanine, Mc) under different chemical environments, which can be used to study the mechanism of antimicrobial activity. Peptides 1-3 exhibit a marked decrease in antimicrobial activity with increasing alkyl chain length. This is likely due to the Sp-Mc isomers in different polar environments forming different aggregate sizes in TBS, as demonstrated by time-dependent dynamic light scattering (DLS). Moreover, peptides 1-3 exhibited low cytotoxicity and hemolytic activity. These probe-modified peptides may provide a novel approach to study the effect of structural changes on antibacterial activity, thus facilitating the design of new antimicrobial agents to combat bacterial infection.

SUBMITTER: Chen L

PROVIDER: S-EPMC4215325 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature

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Synthesis and antibacterial activities of antibacterial peptides with a spiropyran fluorescence probe.

Chen Lei L Zhu Yu Y Yang Danling D Zou Rongfeng R Wu Junchen J Tian He H

Scientific reports 20141031

In this report, antibacterial peptides 1-3 were prepared with a spiropyran fluorescence probe. The probe exhibits a change in fluorescence when isomerized from a colorless spiro-form (spiropyran, Sp) to a colored open-form (merocyanine, Mc) under different chemical environments, which can be used to study the mechanism of antimicrobial activity. Peptides 1-3 exhibit a marked decrease in antimicrobial activity with increasing alkyl chain length. This is likely due to the Sp-Mc isomers in differen ...[more]

PMID: 25358905

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