A clathrin coat assembly role for the muniscin protein central linker revealed by TALEN-mediated gene editing.

ABSTRACT: Clathrin-mediated endocytosis is an evolutionarily ancient membrane transport system regulating cellular receptivity and responsiveness. Plasmalemma clathrin-coated structures range from unitary domed assemblies to expansive planar constructions with internal or flanking invaginated buds. Precisely how these morphologically-distinct coats are formed, and whether all are functionally equivalent for selective cargo internalization is still disputed. We have disrupted the genes encoding a set of early arriving clathrin-coat constituents, FCHO1 and FCHO2, in HeLa cells. Endocytic coats do not disappear in this genetic background; rather clustered planar lattices predominate and endocytosis slows, but does not cease. The central linker of FCHO proteins acts as an allosteric regulator of the pri

SUBMITTER: Umasankar PK 

PROVIDER: S-EPMC4215538 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature