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High lipid storage in vacoular forms of subtype 6 Blastocystis sp. in ostrich.

ABSTRACT: Blastocystis sp., a widely prevalent intestinal protozoan parasite is found in a wide range of animals, including humans. The possibility of zoonotic transmission to human from birds especially ostriches led us to investigate on the cross infectivity of Blastocystis sp. isolated from the ostrich feces as well as the phenotypic and subtype characteristics. There is a need to investigate this especially with the rising number of ostrich farms due to the growing global ostrich industry.100% of the ostriches were found to be positive for Blastocystis sp. using the in-vitro cultivation method. Transmission electron microscopy revealed high electron dense material in the central body of the vacoular forms. The membrane layer of the ostrich isolate was significantly (p?=?0.003) thicker as compared to human isolate. Sudan staining revealed that this was lipid accumulation. We provide evidence for the first time, the existence of subtype 6 which has been previously reported only in pigs and cattle. Cysts, ranging from 3.0 to 7.0 ?m in diameter caused experimental infection in Sprague Dawley rats implicating that Blastocystis sp. isolated from ostriches exhibits low host specificity.The study for the first time demonstrates that Blastocystis sp. subtype 6 do exist in ostriches and show high lipid storage in the vacuoles of the parasites. The study further provides evidence for potential zoonotic transmission in ostrich farms as Blastocystis subtype 6 can infect rats and the same subtype have been previously reported in humans.

SUBMITTER: Chandrasekaran H

PROVIDER: S-EPMC4222385 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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High lipid storage in vacoular forms of subtype 6 Blastocystis sp. in ostrich.

Chandrasekaran Hemalatha H Govind Suresh Kumar SK Panchadcharam Chandrawathani C Bathmanaban Premaalatha P Raman Kalyani K Thergarajan Gaythri G

Parasites & vectors 20141030

<h4>Background</h4>Blastocystis sp., a widely prevalent intestinal protozoan parasite is found in a wide range of animals, including humans. The possibility of zoonotic transmission to human from birds especially ostriches led us to investigate on the cross infectivity of Blastocystis sp. isolated from the ostrich feces as well as the phenotypic and subtype characteristics. There is a need to investigate this especially with the rising number of ostrich farms due to the growing global ostrich in ...[more]

PMID: 25358755

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Project description:Blastocystis sp. is a commonly reported intestinal parasite with a worldwide distribution. Phylogenetic analyses describe at least 17 subtypes for this parasite, and nine of them have been found in humans. However, the prevalence and some epidemiological characteristics of this parasitic infection in rural communities are not well known. The objective of this cross-sectional study was to evaluate the prevalence, subtypes, and epidemiological factors related to Blastocystis sp. Infection in children from of a small rural community in the central area of Panama. For this, 66 fecal samples from children (1 to 12?years old), were initially analyzed for the presence of parasites by a formalin-ethyl acetate/concentration method. Molecular detection and identification of Blastocystis sp. subtypes were carried out by amplification and sequencing of a partial fragment of the small-subunit ribosomal RNA gene. Using data from a questionnaire, analyses of epidemiological conditions potentially associated with Blastocystis sp. transmission were also conducted. Microscopic diagnostics showed that 33.3% (22/66) of the analyzed samples presented entero-parasites. Among them, Blastocystis sp. was the most prevalent, with 21.2% (14/66), followed by the E. histolytica/dispar/moshkovskii complex 4.5% (3/66), Giardia lamblia 1.5% (1/66) and Strongyloides stercoralis 1.5% (1/66). PCR-based analyses detected a prevalence of Blastocystis sp. infection of 74.2% (49/66) in apparently healthy children. Phylogenetic analysis revealed two different subtypes of this parasite: ST1 with 42.2% (28/66) infected, and ST3 with 31.8% (21/66) infected. In addition, recent diarrhea was significantly associated with Blastocystis sp. infection. None of the other risk factors evaluated was statistically associated with infection. These results highlight the need to further investigate clinical, epidemiological, and genetic characteristics of Blastocystis sp. infections in this community.

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High lipid storage in vacoular forms of subtype 6 Blastocystis sp. in ostrich.

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High lipid storage in vacoular forms of subtype 6 Blastocystis sp. in ostrich.

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