Ontology highlight
ABSTRACT:
SUBMITTER: Halim MA
PROVIDER: S-EPMC5031736 | biostudies-literature | 2016 Sep
REPOSITORIES: biostudies-literature
Halim Mohammad A MA Girod Marion M MacAleese Luke L Lemoine Jérôme J Antoine Rodolphe R Dugourd Philippe P
Journal of the American Society for Mass Spectrometry 20160610 9
Herein we report the successful implementation of the consecutive and simultaneous photodissociation with high (213 nm) and low (10.6 μm) energy photons (HiLoPD, high-low photodissociation) on ubiquitin in a quadrupole-Orbitrap mass spectrometer. Absorption of high-energy UV photon is dispersed over the whole protein and stimulates extensive C-Cα backbone fragmentation, whereas low-energy IR photon gradually increases the internal energy and thus preferentially dissociates the most labile amide ...[more]