Ontology highlight
ABSTRACT:
SUBMITTER: Leighton GO
PROVIDER: S-EPMC4222973 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
PloS one 20141106 11
The reaction of DNA transposition begins when the transposase enzyme binds to the transposon DNA. Sleeping Beauty is a member of the mariner family of DNA transposons. Although it is an important tool in genetic applications and has been adapted for human gene therapy, its molecular mechanism remains obscure. Here, we show that only the folded conformation of the specific DNA recognition subdomain of the Sleeping Beauty transposase, the PAI subdomain, binds to the transposon DNA. Furthermore, we ...[more]