Ontology highlight
ABSTRACT:
SUBMITTER: Yao X
PROVIDER: S-EPMC4223039 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
PloS one 20141106 11
Membrane proteins play key roles in biology. Determination of their structure in a membrane environment, however, is highly challenging. To address this challenge, we developed an approach that couples hydrogen/deuterium exchange of membrane proteins to rapid unfolding and detection by solution-state NMR spectroscopy. We show that the method allows analysis of the solvent protection of single residues in liposome-embedded proteins such as the 349-residue Tom40, the major protein translocation po ...[more]